Research areas: Mycological Laboratory of the Department of Mycology allows its employees to perform isolation of fungal strains and cultures on artificial media under aseptic conditions using a UV light and laminar chamber. Various techniques, with the utilization of alcohol and sodium hypochlorite for surface sterilization of fungal material, are used and liquids such as Purosept for the room sterilization.
In order to obtain cultures, subjected later to the molecular testing, the isolation of specific fungi species is carried out. An isolation of endophytes of selected plant species such as Juncus trifidus, Dryas octopetala is also performed, together with the isolation of fungi from the bacterial bio-films and from the air with the use of MAS 100 Eco device. Specific isolation techniques with the use of NaCl and Tween 20 are used for the isolation of black yeasts from the rocks.
Laboratory studies are used in both, research of taxonomic and ecological profile and also in the handling of the City Hall of Krakow and the National Museum (performing mycological expertises).
- Automatic pipette of variable volume
- Pipette tips for single use;
- Eppendorf tubes with a volume of 1.5
- Glassware: cylinders, beakers, dishes, flasks, baguettes, bottles;
Purpose: storage of cultures and reagents
B. -200C freezer
Purpose: storing files, reagents, DNA, PCR products, enzymes
C. incubator for fungal cultures MIR 153
Laminar chamber with vertical air flow and UV lamp:
Purpose: work in a sterile conditions, safe work with the fungal cultures
Prestige Medical: sterilization of media and laboratory equipment
Samsung sterilizer: sterilization of media and water
OHAUS electronic laboratory scales
Purpose: Precise measurement of material with readability of 1 mg
Purpose: mixing prepared solutions with a maximum volume of 0.5 l
Purpose: Room sterilization
MAS 100 Eco Sampler
Application: air sampling
Thermometer and a pH pole meter
Purpose: measuring parameters in the field